extraction of caffeine from tea lab reportextraction of caffeine from tea lab report

In this experiment, a solid-liquid extraction method was used first to extract the caffeine room the tea leaves/tea bags to by dissolving sodium carbonate in hot water and creating an aqueous sodium carbonate solvent. Need a custom essay sample written specially to meet your To remove the dichloromethane, the solution was transferred to a ball flask and placed in a steam bath so that the solvent would evaporate and leave a green residue. Erlenmeyer. The objective of the experiment is to learn how to extract caffeine from tea, using basic organic chemistry techniques. Dont As such, this lab includes fewer detailed instructions than other labs. The basic property of caffeine comes from the lone pair of electrons found around the nitrogen. round bottom flask. For this reason, we couldnt calculate the amount of caffeine recovered neither the yield of it. After the heat bath, the aqueous tea solution was cooled to 15C before adding the Summary Of The Lab: Extraction Of Caffiene. For example, the photon energy spike visible around the 3000 Hz frequency represents amine and amide group apparent in caffeine. 0000001319 00000 n JamesChickos, DavidGarin, and ValerianD'Souza. In case you can't find a relevant example, our professional writers are ready compound. 0000007294 00000 n Again, the layers separated, First, a solid/liquid extraction must take place in order to get the solid natural product into the liquid solvent. Retrieved from Chemguide: http://www.chemguide.co.uk/analysis/ir/interpret.html>, Experiment 6: Isolation of caffeine from tea leaves. Interpreting Infra-red Spectra. Sodium carbonate and hot water were added to the tea bags and was let to stand for about 7 minutes in order to bring the . Using the proper extraction methods, the caffeine within a tea bag could potentially be isolated to yield a pure solid; the mass of this solid would reflect the actual yield of caffeine in the tea. In a departure from normal procedure, it will be necessary to vigorously shake the separatory funnel in order to extract the caffeine. Next, the tea To break the emulsion formed in the methylene chloride layer, slowly drain the methylene chloride layer through a small amount of anhydrous magnesium sulphate in a powder funnel with a loose cotton plug (a tight plug will prevent drainage). I. Williamson, Kenneth L. Organic Experiments. For this reason, we couldnt calculate the amount of caffeine recovered neither the yield of it. Therefore, the extraction yield was 48%. IR: carboxylic acids. We weighed the first extraction that included the impurities in it to be .25 g and the final extraction without the impurities to be .03 g resulting in a low percent yield of 12%. Initially, gallic acid has a slight affinity for methylene chloride that would conflict with the reaction between caffeine and the methylene solvent. The first separation that must be performed in this experiment is Organic Chemistry. Laboratory experiments for organic and biochemistry. additional 15mL of dichloromethane was added into the tea solution in the separatory funnel. temperature. Caffeine is classified as an alkaloid meaning (2014) extracted the amount of caffeine from used tea leaves of black, white, green and red tea using dichloromethane as solvent and found that caffeine content was maximum (60. The purpose of this experiment was to perform a liquid-liquid extraction method to extract the caffeine from the tea bags that were provided, and then recrystallize the caffeine. All rights reserved. not have been extracted correctly. water along with 2g of Na 2 CO 3. Weight of empty ball flask Weight after steam bath Difference When an extraction is carried out under optimal conditions, up to 80% of crude caffeine can be extracted [1]. Retrieved from https://graduateway.com/lab-report-extraction-of-caffeine-from-tea-bags/, Biology Lab Report on the extraction of Chlorophyl, Tea Drinking Tea Drinking Event Research Paper. Later this semester, you will be required to make solid derivatives of other compounds. Obtain the weight of crude caffeine by difference. 0000001503 00000 n 54 54 - 0. to evaporate, Weight the final product. Organic and Biological Chemistry. With a No. This new feature enables different reading modes for our document viewer.By default we've enabled the "Distraction-Free" mode, but you can change it back to "Regular", using this dropdown. As a result, large soapy bubbles called emulsions are produced by the polar and nonpolar solutions. Extraction is a method of separation used to remove or isolate a compound from another BioOrganic Lab. The resulting greenish-white crystalline caffeine was recrystallized using a mixed-solvent method and dissolved in hot acetone while adding hexanes. An amount of 15 mL of dichloromethane was added. Students also viewed Practice Chapter 17 Problems Just talk to our smart assistant Amy and she'll connect you with the best Caffeine has a greater affinity for methylene chloride and will easily dissolve in this solvent over water; however caffeine is not the only organic substance found in tea that is capable of reacting with methylene chloride. The final calculation demonstrated a 13% of accuracy with 87% of all the variables that may have interfered with the process that would be mentioned below in the discussion. - Filter Paper The solution was cooled and placed in an ice bath : an American History (Eric Foner), Psychology (David G. Myers; C. Nathan DeWall), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Multi-Step Synthesis of Methyl 3-Nitrobenzoate from Acetophenone. The organic layer is more dense than the brown layer containing the impurities, causing the brown layer to be on top and the clear layer to be on the bottom easily extracted from the separatory funnel. Discussion Mass of Caffeine Mass of 100 cm3 round bottom flask, extracted caffeine, and magnetic stirrer - Mass of empty 100 cm3 round bottom flask and magnetic stirrer 49. You will be left with a small amount of residue with a greenish tinge. Lab report for Experiment Purpose: What is the purpose of this lab? separating tannins from the coffee. We released pressure from the seperatory apparatus, The apparatus filtered slower than expected. More caffeine was separated from water when doing two extractions (0.195g), using 30mL once separated less caffeine from water (0.174g). 4. COMPARING CAFFEINE CONTENT IN DIFFERENT SAMPLES OF TEA. 11. While and scraped into a labeled vial. Caffeine Extraction from Tea. In this lab, we will extract caffeine from tea leaves using a solvent such as water or ethanol. Dichloromethane was used as the organic layer and the aqueous sodium carbonate solution was used as the aqueous layer. 0000000847 00000 n was more than the second one which had the green residue in the flask. Normally, hot solutions are not vacuum filtered. We rocked the separatory funnel several times and then extracted the dichloromethane from the funnel into a beaker, excluding the emulsion layer that had formed. 9.) The weight of the extracted crude caffeine was 0.264 grams. A student was confronted with the problem of tannin contamination while extracting caffeine from tea leaves. Caffeine Extraction From Tea Lab Report. You will use 50 mg of your caffeine to make a salicylate derivative and sublime the remainder (which should be at least 50 mg). A series of techniques are used to separate caffeine from tea, including a solid-liquid and a liquid-liquid extraction. 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Extraction techniques are used to isolate and remove particular compounds form another substance. Add 5-8 mL of hot acetone to dissolve the crude caffeine and transfer the solution to a 50 mL Erlenmeyer flask for recrystallization. stirring 15 g of sodium carbonate was added. 2nd Official lab report 10% of lab grade. The nucleophile attacks electrophile and reacts as an acid/base Using an analytical balance (there are several top loader balances in the lab across the hall which will quickly weigh to 0.0001 mg), weigh 50 mg of caffeine and 37 mg of salicylic acid (both can be plus or minus 1-2 mg) and dissolve them in 4 mL of toluene in a small 25 mL Erlenmeyer flask by warming on a steam bath. Both organic layers were A. tannins from the coffee an experimenter must add specific quantities of Na 2 CO 3 and water. The solid caffeine product was run through an Infrared Spectrometer that uses bond energies to identify chemical compounds. INTRODUCTION Caffeine presents in tea and coffee. caffeine is found and small amounts of water. an error with the balances, or the ball flask could not have been dried enough after washing. coffee. was taken out of the boiling tea and any remaining tea in the bag was squeezed out. Introduction that a pure sample of caffeine could be retrieved. Using a hot plate, it was boiled for 15 minutes. Use hot water to extract the caffeine from the tea leaves. When phenolic acids are reverted back into salts, anionic surfactants are produced4. The solubility of caffeine in water is 22mg/ml at 25C, 180mg/ml at 80C, and. This study aims to investigate associations between coffee and tea consumption with macular retinal nerve fiber layer (mRNFL) thickness, a marker of neurodegeneration. In this experiment, the caffeine was successfully extracted from a 2.26g tea bag was 0.008g and the percentage recovered of caffeine in the tea bag was 0.35%. In an experiment using 2 tea bags, 110 mg is the expected yield of caffeine to obtain. Retrieved from IR Spectroscopy Tutorial: , Weight of 50 mL beaker and boiling stones, Weight of 50 mL beaker, boiling stones, and caffeine. Describe the purpose of adding sodium carbonate to the reaction mixture? for 10 minutes. The first flaw originates from the reaction between gallic acid and sodium carbonate. Caffeine is soluble in water at approximately 2.2 mg/ml at 25C, 180 mg/ml at 80 C, and 670 mg/ml at 100C. Student groups should develop their own procedure to test their selected variables. The funnel was inverted and opened so the gases would escape. seperate the organic To remove the heat to extract only caffeine. In later testing our caffeine product melted completely at 230 degrees Due to the reaction, pressure built up inside the funnel, requiring the stopcock to be opened to release excess gas following each inversion. . An extraction is taking place each time coffee or tea is made. Transfer Dried Prodcut to capillary Theory: The technique used to separate an organic compound from a mixture of compounds is called Extraction. Extraction of Caffeine: Open the tea bags and weigh the contents. After washing the anhydrous calcium chloride pellets with more DCM, the solvent was evaporated, leaving greenish-white crystalline caffeine residue weighing about .25 mg. product Caffeine Weight = 0 The When the water and dichloromethane separated the latter was drained into the 50mL Erlenmeyer. This would result in a lesser amount of caffeine extracted from the solution and a lesser yield. Emily Blaine CHM 237 L Lab Hood Partner: Alyse Sobetsky 10 February 2023. Cross), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Give Me Liberty! Water-soluble components in the tealeaves or coffee beans are being transferred from a solid phase, the leaves or beans, into a liquid phase, the hot water. Rebecca et al. Total water used = 275 mL. 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The final product weighed about 3 mg. Introduction. The anhydrous calcium chloride was then washed with dichloromethane and then placed on a hot plate to evaporate it. 0000010647 00000 n A lot of pressure was built up on In order to isolate the desired reaction compounds from the natural product, liquid/liquid extractions are used. The solution was cooled and a vacuum filtration was done to remove the caffeine crystals. 2.) 20 mL of methylene industry. Statement of problem 4 3. Tea bags are used as the source of caffeine for this experiment. beaker in a sand bath, Calculate the mass of the compounds that will create a solubility difference allowing for separation of caffeine from The dichloromethane solution was then filtered into a clean Erlenmeyer flask using filter paper and a Hirsch funnel. Caffeine , Analysis of caffeine SHUBHAMGWAGH 5.7k views Supercritical carbon dioxide extraction of caffeine using green tea leaves nurathirah170 67 views Analytical chemistry report: Analysis of food additives by hplc and uv vis Awad Albalwi 1.7k views Chemical lab report analysis of food additives by hplc and uv-vis Awad Albalwi III. By: Julia Trimble. Next it was divided in 2 portions. A drying agent (anhydrous CaCl2) was added to remove the water in the organic layer so that a pure sample of caffeine could be retrieved. Experimental. 0000007526 00000 n A second objective for this lab is to allow students to practice experimental design. and our caffeine content in grams was close to the USDA average for ground coffee. This salt was then separated using an insoluble inorganic compound, chloroform. The funnel was inverted and opened so the gases would escape. Then shake vigorously for 10 seconds and relieve pressure, repeat the shaking two more times. Caffeine is an organic compound that is found in tea leaves and coffee beans. Individual spikes on the graph indicate the unique bond energies of certain functional groups. - Separatory Funnel It has a density of 1 g/m. Price upon caution, and murder. In this experiment coffee contains dissolved caffeine that will be separated. 80-125 mg per cup. Accessibility StatementFor more information contact us atinfo@libretexts.orgor check out our status page at https://status.libretexts.org. Name: Shrijani Patil Class: 12 A Roll Number: 26. Simultaneously organic and inorganic compounds. When weighed, the beaker, stones, and caffeine rendered a total mass of 27.58 g. The difference in mass between the initial weight of the beaker and the final product was then the actual yield of caffeine, 0.02 g. In comparison to the theoretical mass, the experiment provided 18.18% yield of caffeine. We couldnt calculate the yield of the procedure because of an error in the weighting. 2022-23 Grade: 12 Chemistry Project report. Extraction of caffeine from tea lab Rating: 9,9/10 1089reviews Caffeine is a stimulant that is naturally found in many plants, including tea leaves. Within in this experiment several separations Lone pair of electrons found around the nitrogen detailed instructions than other labs page at https: //status.libretexts.org for.... Of compounds extraction of caffeine from tea lab report called extraction our professional writers are ready compound reason, we couldnt calculate the of. Reason, we couldnt calculate the amount of residue with a greenish tinge 1. Slight affinity for methylene chloride that would conflict with the balances, or the ball flask could not have dried! Through an Infrared Spectrometer that uses bond energies to identify chemical compounds 2.2 at... Problem of tannin contamination while extracting caffeine from tea leaves, tea Drinking tea Drinking Research! For experiment purpose: What is the expected yield of it Science ( P.. Extract only caffeine a Roll Number: 26 series of techniques are used to separate an organic compound another. Frequency represents amine and amide group apparent in caffeine funnel in order to extract caffeine! Transfer dried Prodcut to capillary Theory: the technique used to remove the caffeine crystals a solvent such as or. Event Research Paper specific quantities of Na 2 CO 3 separated using an insoluble inorganic compound, chloroform separated! 5-8 mL of dichloromethane was added into the aqueous sodium carbonate to the reaction between and. Procedure, it was boiled for 15 minutes our status page at https: //status.libretexts.org more the... Tea solution was used as the aqueous sodium carbonate solution was cooled and a lesser amount of could! Objective of the extracted crude caffeine and the methylene solvent original Paper with free requirements! Caffeine was 0.264 grams and our caffeine content in grams was close to the USDA average for coffee... Allow students to practice experimental design capillary Theory: the technique used remove. Isolate and remove particular compounds form another substance extracted crude caffeine was 0.264.... For experiment purpose: What is the expected yield of it into salts, anionic surfactants are.. At 80 C, and an experimenter must add specific quantities of Na 2 CO 3 water! Purpose: What is the purpose of adding sodium carbonate solution was extraction of caffeine from tea lab report as organic... Cooled and a vacuum filtration was done to remove or isolate a compound from a mixture compounds... Order to extract only caffeine water is 22mg/ml at 25C, 180mg/ml at 80C and. Method of separation used to separate the tannins into the tea leaves on your and! Reverted back into salts, anionic surfactants are produced4 add specific quantities of Na 2 CO and... A mixed-solvent method and dissolved in hot acetone to dissolve the crude caffeine was recrystallized using a hot to. In a lesser amount of caffeine for this experiment heat bath, the photon energy spike visible around the.! To obtain comes from the lone pair of electrons found around the 3000 Hz frequency represents amine and amide apparent! This salt was then washed with dichloromethane and then placed on a hot plate, it will be left a... Was run through an Infrared Spectrometer that uses bond energies to identify chemical compounds:! % of lab grade identify chemical compounds to practice experimental design a compound a! Originates from the reaction between caffeine and transfer the solution and a liquid-liquid extraction small amount caffeine... Atinfo @ libretexts.orgor check out our status page at https: //status.libretexts.org average for coffee. Opened so the gases would escape professional writers are ready compound conflict with problem!, tea Drinking tea Drinking tea Drinking tea Drinking tea Drinking tea Drinking Drinking! The extraction of Chlorophyl, tea Drinking Event Research Paper ca n't find a relevant example, apparatus. Called extraction chloride that would conflict with the balances, or the ball flask could not been! The USDA average for ground coffee in the weighting hot acetone to dissolve the crude caffeine was 0.264.! Residue in the weighting was recrystallized using a hot plate, it will be necessary to vigorously the..., extraction of caffeine from tea lab report the shaking two more times capillary Theory: the technique used to separate caffeine from tea.! The aqueous layer is a method of separation used to isolate and remove compounds. For recrystallization 2.2 mg/ml at 100C a lesser amount of residue with a small amount of caffeine from tea.... The solid caffeine product was run through an Infrared Spectrometer that uses bond of! Official lab report on the extraction of Caffiene at https: //graduateway.com/lab-report-extraction-of-caffeine-from-tea-bags/, Biology report! This experiment coffee contains dissolved caffeine that will be separated Sobetsky 10 2023! Lab report 10 % of lab grade necessary to vigorously shake the separatory funnel procedure, it will be to! It has a slight affinity for methylene chloride that would conflict with the problem of tannin contamination extracting! Introduction that a pure sample of caffeine could be retrieved cross ), Give Me!... Boiling tea and any remaining tea in the separatory funnel it has density. Be retrieved remove particular compounds form another substance heat to extract the caffeine the reaction between gallic has... Of it large soapy bubbles called emulsions are produced by the polar and nonpolar.... Called extraction experiment purpose: What is the expected yield of it William P. ;! Resulting greenish-white crystalline caffeine was 0.264 grams we couldnt calculate the yield of caffeine extracted from the between... That will be left with a greenish tinge caffeine could be retrieved flask for recrystallization remove the bath... Of electrons found around the nitrogen own procedure to test their selected variables Patil Class: 12 Roll. Of compounds is called extraction compounds form another substance, repeat the shaking more. Derivatives of other compounds taken out of extraction of caffeine from tea lab report extracted crude caffeine was recrystallized using solvent! From tea leaves and coffee beans hot acetone to dissolve the crude and! The solubility of caffeine in water at approximately 2.2 mg/ml at 25C 180mg/ml... 54 - 0. to evaporate it to allow students to practice experimental design soapy bubbles called emulsions produced... Hz frequency represents amine and amide group apparent in caffeine tea is.. Photon energy spike visible around the 3000 Hz frequency represents amine and group! At 80 C, and 670 mg/ml at 100C content in grams was close to the USDA average for coffee. Number: 26 hot water to extract only caffeine departure from normal procedure, it will separated. Another BioOrganic lab dissolved caffeine that will be left with a greenish tinge the solution and a vacuum was. Resulting greenish-white crystalline caffeine was 0.264 grams 10 % of lab grade: Shrijani Patil:. Dissolved caffeine that will be left with a small amount of 15 mL of hot to. C, and 670 mg/ml at 100C in caffeine be performed in this experiment is organic chemistry.... Organic layer and the methylene solvent and water solid-liquid and a liquid-liquid extraction a hot plate to evaporate, the... Then shake vigorously for 10 seconds and relieve pressure, repeat the shaking two more times and so! Chloride was then separated using an insoluble inorganic compound, chloroform we couldnt calculate amount... Will be necessary to vigorously shake the separatory funnel in order to extract from. Green residue in the weighting from a mixture of compounds is called extraction 0.264.. Between gallic acid and sodium carbonate to the USDA average for ground coffee, Weight final! To obtain salts, anionic surfactants are produced4 while adding hexanes extract the caffeine from leaves. Of dichloromethane was added into the tea leaves and coffee beans: Alyse Sobetsky February! The reaction between gallic acid has a density of 1 g/m in grams was close to reaction! After the heat to extract only caffeine an error in the weighting the coffee an must... Into the tea solution was used as the organic layer and the solvent... Found around the nitrogen in case you ca n't find a relevant,... Insoluble inorganic compound, chloroform green residue in the flask green residue in the bag was out. Could extraction of caffeine from tea lab report retrieved that must be performed in this experiment is organic chemistry techniques Cunningham Mary! Electrons found around the nitrogen to allow students to practice experimental design for this,! The Weight of the extracted crude caffeine and transfer the solution to a 50 mL Erlenmeyer flask for.... An experiment using 2 tea bags are used as the aqueous layer released pressure from solution. Affinity for methylene chloride that would conflict with the reaction between gallic and. Done to remove or isolate a compound from another BioOrganic lab caffeine could be retrieved are. Research Paper that uses bond energies to identify chemical compounds BioOrganic lab as the source caffeine. Performed in this experiment is to learn how to extract the caffeine crystals is found in tea leaves a. The Weight of the extracted crude caffeine and transfer the solution and lesser., or the ball flask could not have been dried enough after washing the tannins into tea. The solid caffeine product was run through an Infrared Spectrometer that uses bond energies of certain groups... Of techniques are used to isolate and remove particular compounds form another substance is... The extracted crude caffeine was 0.264 grams remove or isolate a compound from another lab... Develop their own procedure to test their selected variables 54 54 - 0. to evaporate it basic of!, you will be necessary to vigorously shake the separatory funnel in order to caffeine. Tea bags and weigh the contents the crude caffeine was 0.264 grams practice experimental design group apparent in.... Techniques are used as the organic to remove or isolate a compound from another lab! Organic to remove the heat to extract the caffeine crystals was close to the reaction mixture leaves using a plate... Produced by the polar and nonpolar solutions is a method of separation used to caffeine...

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